Replication of G Quadruplex DNA
نویسندگان
چکیده
منابع مشابه
FANCJ promotes replication of G-quadruplex DNA structures
Thank you again for submitting your manuscript EMBOJ-2014-88663, "FANCJ promotes replication of G-quadruplex DNA structures." We have now received all the reports from three experts, which I am enclosing copied below. As you will see, the reviewers express some interest in the basic finding of your study, yet they also raise a number of substantive concerns, and issues with the potential to con...
متن کاملIdentification of the DNA-Binding Domains of Human Replication Protein A That Recognize G-Quadruplex DNA
Replication protein A (RPA), a key player in DNA metabolism, has 6 single-stranded DNA-(ssDNA-) binding domains (DBDs) A-F. SELEX experiments with the DBDs-C, -D, and -E retrieve a 20-nt G-quadruplex forming sequence. Binding studies show that RPA-DE binds preferentially to the G-quadruplex DNA, a unique preference not observed with other RPA constructs. Circular dichroism experiments show that...
متن کاملHuman Pif1 helicase is a G-quadruplex DNA-binding protein with G-quadruplex DNA-unwinding activity.
Pif1 proteins are helicases that in yeast are implicated in the maintenance of genome stability. One activity of Saccharomyces cerevisiae Pif1 is to stabilize DNA sequences that could otherwise form deleterious G4 (G-quadruplex) structures by acting as a G4 resolvase. The present study shows that human Pif1 (hPif1, nuclear form) is a G4 DNA-binding and resolvase protein and that these activitie...
متن کاملDNA Replication through G-Quadruplex Motifs Is Promoted by the Saccharomyces cerevisiae Pif1 DNA Helicase
G-quadruplex (G4) DNA structures are extremely stable four-stranded secondary structures held together by noncanonical G-G base pairs. Genome-wide chromatin immunoprecipitation was used to determine the in vivo binding sites of the multifunctional Saccharomyces cerevisiae Pif1 DNA helicase, a potent unwinder of G4 structures in vitro. G4 motifs were a significant subset of the high-confidence P...
متن کاملHuman Rev1 polymerase disrupts G-quadruplex DNA
The Y-family DNA polymerase Rev1 is required for successful replication of G-quadruplex DNA (G4 DNA) in higher eukaryotes. Here we show that human Rev1 (hRev1) disrupts G4 DNA structures and prevents refolding in vitro. Nucleotidyl transfer by hRev1 is not necessary for mechanical unfolding to occur. hRev1 binds G4 DNA substrates with Kd,DNA values that are 4-15-fold lower than those of non-G4 ...
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ژورنال
عنوان ژورنال: Genes
سال: 2019
ISSN: 2073-4425
DOI: 10.3390/genes10020095